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染色质指导的 RNA 合成:从健康、无毒的根癌农杆菌接种物和蚕豆冠瘿组织中分离的染色质的比较。

Chromatin-directed Ribonucleic Acid Synthesis: A Comparison of Chromatins Isolated from Healthy, Avirulent Agrobacterium tumefaciens Inoculated, and Crown-Gall Tumor Tissues of Vicia faba.

机构信息

Department of Plant Pathology, University of Massachusetts, Amherst, Massachusetts 01002.

出版信息

Plant Physiol. 1973 Oct;52(4):368-72. doi: 10.1104/pp.52.4.368.

Abstract

Chromatin was extracted from healthy, avirulent Agrobacterium tumefaciens inoculated, and crown-gall tumor Vicia faba internodes of the same age. Chromatin from crown-gall tissue produced 5 times more RNA per 100 micrograms of DNA than chromatin from the healthy tissue. When template availability was compared using chromatin with saturating amounts of Escherichia coli RNA polymerase, chromatin from crown-gall tissue had 36% more available template than the controls. In addition, when gamma-(32)P-ATP was incorporated into the RNA synthesizing reaction mixture, with saturating amounts of E. coli RNA polymerase, there were twice as many RNA chain starts in tumor as in control tissue.

摘要

从接种了健康无毒的根癌农杆菌的豌豆和具有相同年龄的根癌组织中提取了染色质。与健康组织的染色质相比,来自根癌组织的染色质每 100 微克 DNA 产生的 RNA 多 5 倍。当使用含有饱和量大肠杆菌 RNA 聚合酶的染色质比较模板可用性时,来自根癌组织的染色质比对照有 36%更多的可用模板。此外,当用 γ-(32)P-ATP 将 RNA 合成反应混合物掺入时,用饱和量的大肠杆菌 RNA 聚合酶,在肿瘤组织中 RNA 链的起始数是对照组织的两倍。

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