Niles R M, Mount M S
Department of Plant Pathology, University of Massachusetts, Amherst, Massachusetts 01002.
Plant Physiol. 1974 Sep;54(3):372-3. doi: 10.1104/pp.54.3.372.
Attempts were made to provide proof for the occurrence of cyclic 3',5'-adenosine monophosphate in healthy and crown gall tissues of Vicia faba. Although our purified extracts gave positive readings in the Gilman binding assay for cyclic AMP, they were not digested by a specific cyclic 3',5'-adenosine monophosphate phosphodiesterase from beef heart. The extracts were digested, however, by a partially purified cyclic nucleotide phosphodiesterase from carrot tissue, which attacks both cyclic 2',3'- and 3',5'-nucleotides. The data indicate that the substances detected in the V. faba extracts are perhaps cyclic 2',3'-nucleotides, a possible RNA degradation product.
人们试图为环磷腺苷在蚕豆健康组织和冠瘿组织中的存在提供证据。尽管我们的纯化提取物在吉尔曼环磷腺苷结合试验中给出了阳性读数,但它们不能被来自牛心的特异性3',5'-环磷腺苷磷酸二酯酶消化。然而,这些提取物能被来自胡萝卜组织的部分纯化的环核苷酸磷酸二酯酶消化,该酶能作用于2',3'-环核苷酸和3',5'-环核苷酸。数据表明,在蚕豆提取物中检测到的物质可能是2',3'-环核苷酸,一种可能的RNA降解产物。