Kokkinakis D M, Brooks J L
Department of Plant Sciences, College of Agriculture and Forestry, West Virginia University, Morgantown, West Virginia 26506.
Plant Physiol. 1979 Aug;64(2):220-3. doi: 10.1104/pp.64.2.220.
The oxidation of indole-3-acetic acid by anionic tomato peroxidase was found to be negligible unless reaction mixtures were supplemented with H(2)O(2). The addition of H(2)O(2) to reaction mixtures initiated a period of rapid indole-3-acetic acid oxidation and O(2) uptake; this phase ended and O(2) uptake fell to a low level when the H(2)O(2) was exhausted. The stoichiometry of the reaction, which is highly dependent on enzyme concentration and pH, suggests that H(2)O(2) initiates a sequence of reactions in which indole-3-acetic acid is oxidized.
发现阴离子型番茄过氧化物酶对吲哚 - 3 - 乙酸的氧化作用可忽略不计,除非反应混合物中添加了H₂O₂。向反应混合物中添加H₂O₂会引发一段快速的吲哚 - 3 - 乙酸氧化和O₂吸收期;当H₂O₂耗尽时,这个阶段结束,O₂吸收量降至低水平。该反应的化学计量关系高度依赖于酶浓度和pH值,这表明H₂O₂引发了一系列吲哚 - 3 - 乙酸被氧化的反应。