Department of Biological Sciences, Herbert H. Lehman College of the City University of New York, Bronx, New York 10468.
Plant Physiol. 1971 Oct;48(4):402-6. doi: 10.1104/pp.48.4.402.
An investigation of the effects of ethylene pretreatment on several facets of auxin metabolism in Coleus blumei Benth "Scarlet Rainbow" revealed a number of changes presumably induced by the gas. Transport of indoleacetic acid-1-(14)C in excised segments of the uppermost internode was inhibited by about 50%. Decarboxylation of indoleacetic acid-1-(14)C by enzyme breis was not affected by the pretreatment. Levels of extractable native auxin in upper leaf and apical bud tissue of the pretreated plants were approximately one-half of those present in untreated plants. The rate of formation of auxin from tryptophan by enzyme breis from pretreated plants was approximately one-half that occurring in incubation mixtures containing the enzyme system from untreated plants. The conjugation of indoleacetic acid-1-(14)C in a form characterized chromatographically as indoleacetylaspartic acid was increased 2-fold in the upper stem region of plants pretreated with ethylene.
对乙烯预处理对 Coleus blumei Benth“Scarlet Rainbow”中生长素代谢几个方面的影响进行了调查,结果发现了一些气体可能诱导的变化。乙烯预处理抑制了最上节间切段中吲哚乙酸-1-(14)C 的运输,抑制率约为 50%。吲哚乙酸-1-(14)C 的酶 Breis 脱羧作用不受预处理的影响。预处理植物的上叶和顶芽组织中可提取的天然生长素水平约为未处理植物的一半。来自预处理植物的酶 Breis 从色氨酸形成生长素的速率约为含有未处理植物酶系统的孵育混合物中发生的速率的一半。用乙烯预处理的植物的上茎区域中,吲哚乙酸-1-(14)C 的缀合以色谱上表征为吲哚乙酰天冬氨酸的形式增加了 2 倍。