细胞游离提取物和楔形鸢尾切芽中的萜类生物合成。
Terpene Biosynthesis in Cell-free Extracts and Excised Shoots from Wedgwood Iris.
机构信息
Department of Horticulture, Michigan State University, East Lansing, Michigan 48823.
出版信息
Plant Physiol. 1973 Nov;52(5):416-21. doi: 10.1104/pp.52.5.416.
Excised shoots and cell-free extracts prepared from Wedgwood iris (Iris hollandica Hoog. "Wedgwood") shoots metabolized (14)C-labeled mevalonic acid (MVA). By using cell-free extracts, the (14)C from MVA-1-(14)C was recovered as (14)CO(2), while that from MVA-2-(14)C was recovered as neutral terpenes, acid-hydrolyzable terpenes, or (14)CO(2). Also, under optimal incubation conditions, 12.8 nanomoles R-MVA-2-(14)C was incorporated into neutral terpenes per milligram fresh weight per hour. In contrast, excised shoots incorporated only 0.58 nanomoles R-MVA-2-(14)C per mg fresh weight per hour. Labeled products identified from the cell-free system were squalene, farnesol, geranylgeraniol, and compounds that are converted to farnesol and geranylgeraniol after alkaline hydrolysis. Squalene and a 4,4-dimethylsterol were identified as products from excised shoots but not the terpene alcohols or the alkaline-hydrolyzable compounds.
从威格伍德鸢尾（Iris hollandica Hoog. "Wedgwood"）芽中提取的外植体和无细胞提取物代谢（14）C 标记的甲羟戊酸（MVA）。使用无细胞提取物，MVA-1-（14）C 的（14）C 被回收为（14）CO2，而 MVA-2-（14）C 的（14）C 被回收为中性萜烯、酸可水解萜烯或（14）CO2。此外，在最佳孵育条件下，每毫克鲜重每小时可将 12.8 纳摩尔 R-MVA-2-（14）C 掺入中性萜烯中。相比之下，外植体仅每毫克鲜重每小时掺入 0.58 纳摩尔 R-MVA-2-（14）C。从无细胞系统中鉴定出的标记产物有角鲨烯、法呢醇、香叶基香叶醇以及经碱水解转化为法呢醇和香叶基香叶醇的化合物。角鲨烯和 4,4-二甲基固醇被鉴定为外植体的产物，但不是萜烯醇或可碱水解的化合物。
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