Department of Plant Sciences, University of California, Riverside, California 92502.
Plant Physiol. 1973 Dec;52(6):554-60. doi: 10.1104/pp.52.6.554.
Time course changes were observed in petiole and stem anatomy and cellulase enzyme patterns in bean (Phaseolus vulgaris L.) explants when 10(-5) or 10(-2)m indoleacetic acid in lanolin paste was applied to acropetal cut surfaces in the presence or absence of ethylene. Auxin (10(-2)m) in the presence of ethylene stimulated rapid ordered cell division and dedifferentiation, with ensuing lateral root formation. Auxin (10(-5)m) caused moderate cortical swelling, pit formation in pith parenchyma, and chloroplast development in certain cortical cells. Exogenous ethylene reduced cell division activity and caused cortical cell swelling and separation. Removal of endogenously generated ethylene by mercuric perchlorate resulted in less ordered cell division patterns and no lateral root formation. Auxin treatments enhanced formation of an active acidic pI cellulase, exogenous ethylene-stimulated formation of an active basic pI cellulase. The absence of basic pI cellulase activity by the removal of endogenously generated ethylene suggests a close dependence of basic pI cellulase activity on ethylene.
当羊毛脂糊中的 10(-5)或 10(-2)m 吲哚乙酸施加到顶端切割面时，存在或不存在乙烯，观察到菜豆（Phaseolus vulgaris L.）外植体叶柄和茎解剖结构和纤维素酶模式的时间进程变化。在乙烯存在下的生长素（10(-2)m）刺激快速有序的细胞分裂和去分化，随后形成侧根。生长素（10(-5)m）引起适度的皮层肿胀、髓质薄壁组织中的pit 形成以及某些皮层细胞中的叶绿体发育。外源乙烯降低细胞分裂活性并引起皮层细胞肿胀和分离。通过过氯酸汞去除内源性产生的乙烯导致细胞分裂模式不那么有序，并且没有侧根形成。生长素处理增强了活性酸性 pI 纤维素酶的形成，外源乙烯刺激了活性碱性 pI 纤维素酶的形成。通过去除内源性产生的乙烯去除碱性 pI 纤维素酶活性表明碱性 pI 纤维素酶活性对乙烯的密切依赖。
