Biotechnology Research Center, Taisei Corporation, Akanehama 3-6-2, 275, Narashino, Chiba, Japan.
Plant Cell Rep. 1995 Jan;15(1-2):43-50. doi: 10.1007/BF01690251.
Nojirimycin bisulfite andp-nitrophenyl-1-thio-ß-D-glucoside, potent inhibitors of ß-glucanase, suppressed cortical dissociation and callus growth in rice roots (Oryza sativa L. cv. Sasanishiki) in the presence of 2,4-D, with resultant suppression of the emergence of callus clumps from the interior of root tissues. These sugar analogs inhibited the 2,4-D-induced increase in the elastic and plastic compliance of cell walls but did not affect oxygen uptake by root explants in the presence of 2,4-D. They also inhibited the activities of CM-cellulase, ß-glucosidase and α-arabinosidase in 2 M NaCl-soluble and/or buffer-soluble fractions of crude root extracts. Involvement of glycan hydrolase activities in cell wall dissociation during callus emergence is discussed.
N-（羟甲基）氨基甲酰基-β-D-吡喃葡萄糖苷和对硝基苯-1-硫代-β-D-吡喃葡萄糖苷是β-葡聚糖酶的强抑制剂，在 2,4-D 存在的情况下，它们抑制了水稻根（Oryza sativa L. cv. Sasanishiki）皮层分离和愈伤组织生长，从而抑制了愈伤组织从根组织内部团块的出现。这些糖类似物抑制了 2,4-D 诱导的细胞壁弹性和塑性顺应性的增加，但在 2,4-D 存在的情况下不影响根外植体的氧摄取。它们还抑制了在 2 M NaCl 可溶和/或缓冲液可溶部分粗根提取物中 CM-纤维素酶、β-葡萄糖苷酶和α-阿拉伯糖苷酶的活性。讨论了糖水解酶活性在愈伤组织出现过程中对细胞壁解离的作用。
