Department of Botany, University of Illinois, Urbana, Illinois 61801.
Plant Physiol. 1977 Dec;60(6):933-4. doi: 10.1104/pp.60.6.933.
Acidification of the suspending medium of corn mitochondria (Zea mays L., WF9 x Mo17) from pH 7.5 to pH 6.8 to 6.4 initiates osmotic swelling with the transportable anions citrate, sulfate, and phosphate. Swelling becomes pronounced with a combination of citrate plus sulfate or phosphate. Acidification proves to activate endogenous respiration, which is essentially zero at pH 7.5. The endogenous respiration transports citrate (in the presence of sulfate or phosphate) which then contributes to respiration and the accelerated osmotic swelling. Mersalyl will inhibit the swelling and antimycin inhibits the endogenous respiration. Magnesium appears to reduce the permeability of the membranes under the acid conditions.
将玉米线粒体(Zea mays L.,WF9 x Mo17)悬浮介质的 pH 值从 7.5 降低至 6.8 至 6.4,会引发可转运阴离子柠檬酸、硫酸根和磷酸根引起的渗透性肿胀。柠檬酸与硫酸根或磷酸根联合作用会使肿胀变得明显。酸化可激活内源性呼吸,在 pH 7.5 时内源性呼吸基本为零。内源性呼吸转运柠檬酸(在硫酸根或磷酸根存在的情况下),然后促进呼吸和加速渗透性肿胀。Mersalyl 会抑制肿胀,而抗霉素会抑制内源性呼吸。镁似乎在酸性条件下降低了膜的通透性。