纤细裸藻在生理条件下质体的不可逆丧失
Irreversible Plastid Loss in Euglena gracilis under Physiological Conditions.
作者信息
Cook J R
机构信息
Departments of Zoology and Botany, University of Maine, Orono, Maine 04473.
出版信息
Plant Physiol. 1974 Feb;53(2):284-90. doi: 10.1104/pp.53.2.284.
Irreversible loss of the ability to develop chloroplasts in Euglena gracilis may develop following transfer from organic medium to defined medium. Requirements for the loss include the absence of light and a temperature of 30 C (the optimal temperature for multiplication) although neither darkness alone nor this temperature alone serves as the bleaching agent. The extent of bleaching of a population can approach 100% but depends heavily on the following conditions: the pH of the defined medium and its phosphate content, the age of the parent culture at transfer, and the length of time spent in the defined medium before cell divisions are permitted. Bleaching is not due to loss of nonreplicating proplastids through "dilution out" as cells divide but appears to be a more direct inactivation of chloroplast differentiation from proplastids.
纤细裸藻从有机培养基转移至特定培养基后，可能会出现叶绿体发育能力的不可逆丧失。这种丧失需要满足无光条件以及30℃的温度（该温度为繁殖的最佳温度），尽管单独的黑暗或该温度本身都不能作为漂白剂。群体的漂白程度可接近100%，但在很大程度上取决于以下条件：特定培养基的pH值及其磷酸盐含量、转移时亲代培养物的年龄，以及在允许细胞分裂前在特定培养基中停留的时间。漂白并非由于细胞分裂时非复制性前质体通过“稀释”而丧失，而是似乎是前质体叶绿体分化的更直接失活。
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