纤细裸藻Z株中S-腺苷甲硫氨酸-镁原卟啉甲基转移酶的研究。
Studies on S-adenosylmethionine-magnesium protoporphyrin methyltransferase in Euglena gracilis strain Z.
作者信息
Ebbon J G, Tait G H
出版信息
Biochem J. 1969 Feb;111(4):573-82. doi: 10.1042/bj1110573.
Abstract
- An enzyme that methylates magnesium protoporphyrin was detected in extracts of light-grown and dark-grown cells of Euglena gracilis. The activity in light-grown cells is two to three times that in cells grown in the dark. 2. The activity is mainly located in the chloroplast fraction from light-grown cells and in proplastids in dark-grown cells. However, in cells grown either in the light or dark, about 15-20% is found in particle-free supernatant. 3. The chloroplast methylating enzyme was solubilized by the action of Tween 80 and partially purified. The properties were investigated. 4. From experiments in which etiolated cells were illuminated in the presence of inhibitors of chloroplast or cytoplasmic protein synthesis, it appears that the methylating enzyme is made on cytoplasmic ribosomes.
摘要
- 在纤细裸藻的光照培养细胞和黑暗培养细胞提取物中检测到一种能使镁原卟啉甲基化的酶。光照培养细胞中的活性是黑暗培养细胞中的两到三倍。2. 该活性主要位于光照培养细胞的叶绿体部分以及黑暗培养细胞的前质体中。然而，无论在光照还是黑暗条件下培养的细胞中，约15 - 20%存在于无颗粒上清液中。3. 叶绿体甲基化酶通过吐温80的作用溶解并部分纯化。对其性质进行了研究。4. 通过在叶绿体或细胞质蛋白质合成抑制剂存在的情况下对黄化细胞进行光照的实验表明，甲基化酶是在细胞质核糖体上合成的。
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