Seirei-gakuen Hamamatsu Junior College of Hygiene and Nursing, Mikatagahara-cho, Hamamatsu 433, Shizuoka, Japan.
Plant Physiol. 1974 Apr;53(4):649-52. doi: 10.1104/pp.53.4.649.
Recently, we isolated dehydroipomeamarone, a new sesquiterpenoid from sweet potato (Ipomoea batatas Lam.) root tissue infected with Ceratocystis fimbriata (Ell. et Halst.). The purpose of this investigation was to determine whether dehydroipomeamarone was a precursor in the biosynthetic pathway of ipomeamarone. The incorporation of acetate-2-(14)C into ipomeamarone was markedly inhibited by the presence of dehydroipomeamarone. Radioactive dehydroipomeamarone was efficiently converted into ipomeamarone, and the compound was biosynthesized earlier than ipomeamarone according to a time course analysis of the production of the terpenoid. These results support the notion that dehydroipomeamarone is an immediate precursor of ipomeamarone. On the other hand, the production of ipomeamarone was slightly lessened in the presence of dehydroipomeamarone. Thus, the marked reduction of acetate-2-(14)C incorporation into ipomeamarone by dehydroipomeamarone may result from both isotopic dilution and an inhibitory effect by exogenous dehydroipomeamarone.
最近，我们从感染 Ceratocystis fimbriata (Ell. et Halst.)的甘薯（Ipomoea batatas Lam.）根组织中分离出了一种新的倍半萜类化合物脱氢甘薯酮。本研究的目的是确定脱氢甘薯酮是否是甘薯酮生物合成途径中的前体。存在脱氢甘薯酮时，醋酸盐-2-（14）C 掺入甘薯酮的明显受到抑制。放射性脱氢甘薯酮可有效地转化为甘薯酮，根据萜类化合物产生的时间过程分析，该化合物的生物合成早于甘薯酮。这些结果支持了脱氢甘薯酮是甘薯酮的直接前体的观点。另一方面，在存在脱氢甘薯酮的情况下，甘薯酮的产量略有减少。因此，脱氢甘薯酮对醋酸盐-2-（14）C 掺入甘薯酮的明显抑制作用可能是由于同位素稀释和外源性脱氢甘薯酮的抑制作用共同导致的。
