Mohsin Sayed Mohammad, Hasanuzzaman Mirza, Parvin Khursheda, Morokuma Masahiro, Fujita Masayuki
Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa, 761-0795, Japan.
Department of Plant Pathology, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh.
World J Microbiol Biotechnol. 2021 Aug 7;37(9):148. doi: 10.1007/s11274-021-03111-5.
Black rot, caused by Ceratocystis fimbriata, is one of the most destructive disease of sweet potato worldwide, resulting in significant yield losses. However, a proper management system can increase resistance to this disease. Therefore, this study investigated the potential of using tebuconazole (TEB) and trifloxystrobin (TRI) to improve the antioxidant defense systems in sweet potato as well as the inhibitory effects on the growth of and antioxidant activity in C. fimbriata. Four days after inoculating cut surfaces of sweet potato disks with C. fimbriata, disease development was reduced by different concentrations of TEB + TRI. Infection by C. fimbriata increased the levels of hydrogen peroxide (HO), malondialdehyde (MDA), and electrolyte leakage (EL), and the activity of lipoxygenase (LOX) by 138, 152, 73, and 282%, respectively, in sweet potato disks, relative to control. In the sweet potato disks, C. fimbriata reduced the antioxidant enzyme activities as well as the contents of ascorbate (AsA) and reduced glutathione (GSH) by 82 and 91%, respectively, compared with control. However, TEB + TRI reduced the oxidative damage in the C. fimbriata-inoculated sweet potato disks by enhancing the antioxidant defense systems. On the other hand, applying TEB + TRI increased the levels of HO, MDA, and EL, and increased the activity of LOX in C. fimbriata, in which the contents of AsA and GSH decreased, and therefore, inhibited the growth of C. fimbriata. These results suggest that TEB + TRI can significantly control black rot disease in sweet potato by inhibiting the growth of C. fimbriata.
由帚状炭疽菌引起的黑腐病是全球甘薯最具毁灭性的病害之一，会导致大幅减产。然而，一套恰当的管理体系可以增强对这种病害的抗性。因此，本研究调查了使用戊唑醇（TEB）和肟菌酯（TRI）来改善甘薯抗氧化防御系统的潜力，以及对帚状炭疽菌生长和抗氧化活性的抑制作用。用帚状炭疽菌接种甘薯块茎切片四天后，不同浓度的TEB + TRI降低了病害的发展。相对于对照，帚状炭疽菌感染使甘薯块茎切片中的过氧化氢（HO）、丙二醛（MDA）和电解质渗漏（EL）水平以及脂氧合酶（LOX）活性分别提高了138%、152%、73%和282%。在甘薯块茎切片中，与对照相比，帚状炭疽菌使抗氧化酶活性以及抗坏血酸（AsA）和还原型谷胱甘肽（GSH）含量分别降低了82%和91%。然而TEB + TRI通过增强抗氧化防御系统降低了接种帚状炭疽菌的甘薯块茎切片中的氧化损伤。另一方面，施用TEB + TRI提高了帚状炭疽菌中HO、MDA和EL的水平，并提高了LOX的活性，其中AsA和GSH的含量下降，因此抑制了帚状炭疽菌的生长。这些结果表明，TEB + TRI可以通过抑制帚状炭疽菌的生长来显著控制甘薯黑腐病。
