Division of Plant Sciences, West Virginia University, Morgantown, West Virginia 26506.
Plant Physiol. 1974 May;53(5):759-63. doi: 10.1104/pp.53.5.759.
Four enzyme-containing fractions were separated by ammonium sulfate fractionation of 2-day, postbreaker tomato (Lycopersicon esculentum L. cv. Manhattan). The pH optima and substrate specificities are reported. The enzymes were identified as a nonspecific beta-glucosidase, an exo-beta-1,4-glucanase, and two endocellulases. Both endocellulase fractions were able to catalyze the hydrolysis of various insoluble cellulose materials.Together, the enzymes studies are capable of completely degrading insoluble cellulose to short chain cellodextrins and glucose. Ripening tomato fruit apears to contain a complex of cellulases similar to that of cellulolytic bacteria and fungi.
采用硫酸铵分级沉淀法从 2 天龄的破碎番茄（Lycopersicon esculentum L. cv. Manhattan）中分离出 4 种含酶的组分。报道了它们的最适 pH 值和底物特异性。这些酶被鉴定为一种非特异性β-葡萄糖苷酶、一种外切β-1,4-葡聚糖酶和两种内切纤维素酶。两种内切纤维素酶都能够催化各种不溶性纤维素材料的水解。研究中的这些酶能够将不溶性纤维素完全降解为短链纤维二糖和葡萄糖。成熟的番茄果实似乎含有类似于纤维素分解细菌和真菌的纤维素酶复合物。
