Department of Agronomy and Range Science and Department of Environmental Horticulture, University of California, Davis, California 95616.
Plant Physiol. 1973 Sep;52(3):278-82. doi: 10.1104/pp.52.3.278.
Barley seeds (Hordeum vulgare L. cv. Himalaya) contain an enzymatic activity which catalyzes the hydrolysis of adenosine cyclic 3': 5'-monophosphate and adenosine cyclic 2': 3'-monophosphate. A large portion of the enzymatic activity is present in the dry seed, existing in both soluble and particulate form. Secretion of the soluble phosphodiesterase from embryoless seeds is enhanced by gibberellic acid and inhibited by abscisic acid, dinitrophenol, and cycloheximide. Attempts to isolate or detect a phosphodiesterase which specifically hydrolyzes adenosine cyclic 3': 5'-monophosphate were unsuccessful. Inhibition experiments indicate that probably one enzyme is involved in the hydrolysis of both of these substrates.
大麦种子(Hordeum vulgare L. cv. Himalaya)含有一种酶活性,可催化腺苷环 3':5'-单磷酸和腺苷环 2':3'-单磷酸的水解。大量的酶活性存在于干种子中,以可溶性和颗粒形式存在。赤霉素可增强无胚种子中可溶性磷酸二酯酶的分泌,而脱落酸、二硝基苯酚和环己亚胺则可抑制其分泌。试图分离或检测专门水解腺苷环 3':5'-单磷酸的磷酸二酯酶的尝试均未成功。抑制实验表明,可能有一种酶参与了这两种底物的水解。