Jacobson B S, Jaworski J G, Stumpf P K
Department of Biochemistry and Biophysics, University of California, Davis, California 95616.
Plant Physiol. 1974 Oct;54(4):484-6. doi: 10.1104/pp.54.4.484.
Stearyl-acyl carrier protein desaturase (EC 1.14.99.6), present in the stroma fraction of spinach (Spinacia oleracea) chloroplasts, rapidly desaturated enzymatically prepared stearyl-acyl carrier protein to oleic acid. No other substrates were desaturated. In addition to stearyl-acyl carrier protein, reduced ferredoxin was an essential component of the system. The electron donor systems were either ascorbate, dichlorophenolindophenol, photosystem I and light, or NADPH and ferredoxin-NADP reductase. The desaturase was more active in extracts prepared from chloroplasts obtained from immature spinach leaves than from mature leaves. Stearyl-acyl carrier protein desaturase also occurs in soluble extracts of avocado (Persea americana Mill.) mesocarp and of developing safflower (Carthamus tinctorius) seeds.
硬脂酰 - 酰基载体蛋白去饱和酶(EC 1.14.99.6)存在于菠菜(Spinacia oleracea)叶绿体的基质部分,能将酶促制备的硬脂酰 - 酰基载体蛋白迅速去饱和生成油酸。没有其他底物被去饱和。除了硬脂酰 - 酰基载体蛋白外,还原型铁氧还蛋白是该系统的必需成分。电子供体系统可以是抗坏血酸、二氯酚靛酚、光系统I和光,或者NADPH和铁氧还蛋白 - NADP还原酶。从未成熟菠菜叶片获得的叶绿体提取物中的去饱和酶比从成熟叶片中获得的更具活性。硬脂酰 - 酰基载体蛋白去饱和酶也存在于鳄梨(Persea americana Mill.)中果皮的可溶性提取物和发育中的红花(Carthamus tinctorius)种子中。