Crafts C B, Miller C O
Department of Plant Sciences, Indiana University, Bloomington, Indiana 47401.
Plant Physiol. 1974 Oct;54(4):586-8. doi: 10.1104/pp.54.4.586.
Several fungi including six species of the genus Rhizopogon, 22 species of Hebeloma and one of Agaricus have been screened for production of cytokinins. The screening was done by culturing cytokinin-requiring soybean callus tissue alongside the fungus on a medium lacking a cytokinin supply. Growth of the soybean callus indicated production of cytokinins by the fungus. Of the fungi tested, only R. ochraceorubens A. H. Smith gave off sufficient cytokinin to be detected. Although a number of mycorrhizal species are now known to make and give off cytokinins, an even larger number apparently do not do so under the conditions of screening employed. An unidentified ectendotrophic species definitely gave off trans-zeatin, which has been crystallized, and probably trans-ribosylzeatin. Suillus punctipes (Pk.) Sing. apparently produced the same two cytokinins.
已经对包括六种须腹菌属、二十二种丝膜菌属和一种蘑菇属在内的几种真菌进行了细胞分裂素生产的筛选。筛选方法是将需要细胞分裂素的大豆愈伤组织与真菌一起培养在缺乏细胞分裂素供应的培养基上。大豆愈伤组织的生长表明真菌产生了细胞分裂素。在所测试的真菌中，只有赭红须腹菌A.H.史密斯释放出足够量的细胞分裂素以便被检测到。虽然现在已知许多菌根物种能够产生并释放细胞分裂素，但显然在所用的筛选条件下，还有更多的菌根物种不会这样做。一种未鉴定的内外生营养物种肯定释放出了已结晶的反式玉米素，可能还有反式核糖基玉米素。点柄乳牛肝菌显然也产生了同样的两种细胞分裂素。
