Department of Botany, University of Georgia, Athens, Georgia 30601.
Plant Physiol. 1975 Jul;56(1):78-82. doi: 10.1104/pp.56.1.78.
A quick procedure for the isolation of nuclei with good yield from soybean hypocotyl (Glycine max var. Wayne) was developed. The isolated nuclei appeared to retain their structural integrity. They were typically ellipsoidal with minima and maxima diameter of about 6 and 8 to 10 micrometers. While the nuclei were similar in size, the nucleoli were significantly larger in nuclei from auxin-treated tissue. The DNA content per nucleus was 4 +/- 1 picograms for both untreated and auxin-treated tissues. The DNA: RNA: protein ratio of isolated nuclei in untreated and auxin-treated tissues was 1: 3.1: 11 and 1: 5.4: 21.7, respectively. The purified nuclei were active in RNA synthesis; the level of RNA polymerase II activity expressed in the nuclei from untreated tissue was 50 to 60% higher than RNA polymerase. I. The nuclei from auxin-treated tissues contained about 2.5 times as much RNA polymerase I activity as nuclei from untreated tissue. The purified nuclei from both untreated and auxin-treated tissues were also active in the incorporation of (3)H-TTP into DNA.
从大豆下胚轴（Glycine max var. Wayne）中快速分离出产量高的细胞核的方法已经建立。分离出的细胞核似乎保持了其结构的完整性。它们通常呈椭圆形，最小和最大直径约为 6 微米和 8 至 10 微米。虽然细胞核的大小相似，但生长素处理组织中的核仁明显更大。每个细胞核的 DNA 含量为 4 ± 1 皮克，无论是未处理还是生长素处理的组织都是如此。未处理和生长素处理组织中分离出的细胞核的 DNA：RNA：蛋白质比率分别为 1：3.1：11 和 1：5.4：21.7。纯化的细胞核在 RNA 合成中具有活性；未处理组织中细胞核表达的 RNA 聚合酶 II 活性比 RNA 聚合酶 I 高 50%至 60%。生长素处理组织中的细胞核中 RNA 聚合酶 I 活性约为未处理组织的 2.5 倍。来自未处理和生长素处理组织的纯化细胞核在将 (3)H-TTP 掺入 DNA 中也具有活性。
