从大豆下胚轴中分离核仁及定位 RNA 聚合酶 I。
Isolation of nucleoli and localization of ribonucleic Acid polymerase I from soybean hypocotyl.
机构信息
Department of Botany, University of Georgia, Athens, Georgia 30602.
出版信息
Plant Physiol. 1975 Dec;56(6):850-2. doi: 10.1104/pp.56.6.850.
Abstract
An effective method for the isolation of nucleoli from auxin-treated soybean (Glycine max, var. Wayne) hypocotyl was developed by polytron homogenization and sucrose gradient centrifugation. The nucleoli expressed only the alpha-amanitin-insensitive RNA synthetic activity. This activity chromatographed as RNA polymerase I on DEAE-cellulose. It appears that the plant nucleolus, like the animal nucleolus, is the site of localization for RNA polymerase I.
摘要
采用匀浆器匀浆和蔗糖梯度离心法，从生长素处理的大豆（Glycine max，var. Wayne）下胚轴中分离核仁。核仁仅表达α-鹅膏蕈碱不敏感的 RNA 合成活性。这种活性在 DEAE-纤维素上作为 RNA 聚合酶 I 进行色谱分离。植物核仁似乎与动物核仁一样，是 RNA 聚合酶 I 的定位部位。
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