Heath R L
Department of Biology, University of California, Riverside, California 92502.
Plant Physiol. 1975 Aug;56(2):181-4. doi: 10.1104/pp.56.2.181.
This paper describes the assembly (from commercially available components) of an antilog converter, which transforms the output signals of ion-specific electrodes to ionic concentrations suitable for a linear recorder. It responds linearly to cation concentrations from 10 mum to at least 10 mm and can be used for electrodes kept at any temperatures (0 to 50 C). The leakage of K(+) from a unicellular algae (Chlorella sorokiniana) can be induced by Triton X-100, heating, or suspension in a tris buffer and is used to demonstrate the operation of this device.
本文描述了一种反对数转换器(由市售组件组装而成),该转换器可将离子特异性电极的输出信号转换为适合线性记录仪的离子浓度。它对10微摩尔至至少10毫摩尔的阳离子浓度呈线性响应,可用于任何温度(0至50摄氏度)下的电极。单细胞藻类(索氏小球藻)中的钾离子泄漏可由曲拉通X-100、加热或悬浮在三羟甲基氨基甲烷缓冲液中诱导产生,并用于演示该装置的运行情况。