淀粉胚乳中一些酶的出现和小麦糊粉层中同工过氧化物酶的激素调节。
Occurrence of some enzymes in starchy endosperm and hormonal regulation of isoperoxidase in aleurone of wheat.
机构信息
New York State Agricultural Experiment Station, Cornell University, Geneva, New York 14456.
出版信息
Plant Physiol. 1975 Dec;56(6):797-800. doi: 10.1104/pp.56.6.797.
Peroxidase, indoleacetic acid-oxidase, alkaline, and acid phosphatases were detected in dry starchy endosperm (minus aleurone) of wheat grain. The isoperoxidase pattern differed in different parts of the dry grain. Several new isoperoxidases were found in embryos after soaking. The intensity of isoperoxidases in aleurones was enhanced in the presence of embryo or 2 muM GA(3) after 24 hours of soaking, but decreased after 72 hours. Indoleacetic acid and kinetin had no effect on isoperoxidase of aleurone. Actinomycin D and cycloheximide had no effect on isoperoxidases of aleurones from embryonectomized or naturally occurring embryoless grains. However, these two inhibitors increased the intensity of isoperoxidases in aleurones of intact embryonated grains after soaking.
在小麦干淀粉胚乳（不含糊粉层）中检测到过氧化物酶、吲哚乙酸氧化酶、碱性和酸性磷酸酶。干谷物不同部位的同工过氧化物酶模式不同。浸泡后在胚中发现了几种新的同工过氧化物酶。在浸泡 24 小时后，胚或 2μM GA（3）存在下，糊粉层的同工过氧化物酶强度增强，但 72 小时后减少。吲哚乙酸和激动素对糊粉层的同工过氧化物酶没有影响。放线菌素 D 和环己酰亚胺对胚缺失或自然发生的无胚粒的糊粉层的同工过氧化物酶没有影响。然而，这两种抑制剂在浸泡后增加了完整有胚粒的糊粉层同工过氧化物酶的强度。
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