Michigan State University-Atomic Energy Commission Plant Research Laboratory, Michigan State University East Lansing, Michigan 48824.
Plant Physiol. 1974 Dec;54(6):813-6. doi: 10.1104/pp.54.6.813.
Cell-free extracts from gibberellic acid-treated barley (Hordeum vulgare L. cv. Himalaya) aleurone layers show phosphorylcholine glyceride transferase activity greater than that from control layers. The increase in activity is not prevented by a mixture of amino acid analogs nor by cordycepin under conditions in which it is demonstrated that the analogs and the cordycepin are entering the cells in effective concentrations. We conclude therefore that the GA(3)-dependent increase in phosphorylcholine glyceride transferase activity (which occurs within the first 4 hours of GA(3) treatment) does not require RNA synthesis or protein synthesis.
用赤霉素(GA3)处理的大麦(Hordeum vulgare L. cv. Himalaya)糊粉层的无细胞提取物显示出比对照层更高的磷酸胆碱甘油酯转移酶活性。在氨基酸类似物混合物和虫草素存在的条件下,活性的增加不能被阻止,在这些条件下,已经证明类似物和虫草素以有效的浓度进入细胞。因此,我们得出结论,GA3 依赖性的磷酸胆碱甘油酯转移酶活性的增加(在 GA3 处理的头 4 小时内发生)不需要 RNA 合成或蛋白质合成。