Radiation Biology Laboratory, Smithsonian Institution, Rockville, Maryland 20852.
Plant Physiol. 1976 Mar;57(3):440-5. doi: 10.1104/pp.57.3.440.
An action spectrum for light-induced carotenoid biosynthesis in Neurospora crassa was determined in 4 to 20 nm steps from 260 to 800 nm. Four-day, dark-grown mycelial pads of N. crassa were exposed to varying amounts of monochromatic radiant energy and time. After a 48-hour incubation period at 6 C, carotenoid content was assayed spectrophotometrically in vivo. The action spectrum has maxima at 450 and 481 nm in the visible range and at 280 and 370 nm in the ultraviolet. A pigment synthesized by Neurospora whose absorption spectrum resembles the action spectrum is beta-carotene.A model for the regulation of carotenoid biosynthesis in N. crassa is proposed which describes a mechanism by which beta-carotene could act as a photoregulator. This carotenoid is suggested to be both photoreceptor for and regulator of carotenoid biosynthesis.
从 260nm 到 800nm,我们以 4nm 到 20nm 的步长,在链孢霉中确定了光诱导类胡萝卜素生物合成的作用光谱。将处于黑暗中生长了 4 天的链孢霉菌丝垫暴露于不同量的单色辐射能和时间下。在 6°C 下孵育 48 小时后,通过分光光度法在体内测定类胡萝卜素含量。作用光谱在可见光谱中有最大值,分别为 450nm 和 481nm,在紫外线光谱中有最大值,分别为 280nm 和 370nm。一种由链孢霉合成的色素,其吸收光谱类似于作用光谱,为β-胡萝卜素。我们提出了一个用于调节链孢霉中类胡萝卜素生物合成的模型,该模型描述了一种β-胡萝卜素可以作为光调节剂的机制。这种类胡萝卜素被认为是类胡萝卜素生物合成的光受体和调节剂。