豌豆（Pisum sativum L.）根瘤中细胞分裂素的鉴定
Identification of Cytokinins of Root Nodules of the Garden Pea, Pisum sativum L.
作者信息
Syõno K, Torrey J G
机构信息
Cabot Foundation, Harvard University, Petersham, Massachusetts 01366.
出版信息
Plant Physiol. 1976 Apr;57(4):602-6. doi: 10.1104/pp.57.4.602.
Abstract
Five cytokinin activities which induced soybean callus proliferation were detected in ethanol extracts of root nodules of the garden pea (Pisum sativum L., cv. Little Marvel). The most active factors among them were identified as zeatin and its riboside on the basis of their mobility on thin layer chromatography in three solvent systems. Smaller activities of zeatin ribotide, isopentenyladenine and its riboside were also detected. Cytokinin activity gradually decreased with the cultivation period, but no qualitative change in the active compounds was found.
摘要
在豌豆（Pisum sativum L.，品种Little Marvel）根瘤的乙醇提取物中检测到了五种诱导大豆愈伤组织增殖的细胞分裂素活性。基于它们在三种溶剂系统中的薄层色谱迁移率，其中最活跃的因子被鉴定为玉米素及其核苷。还检测到了玉米素核糖苷、异戊烯腺嘌呤及其核苷的较小活性。细胞分裂素活性随培养时间逐渐降低，但活性化合物未发现定性变化。
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