菜豆中 8-[C]苄基氨基嘌呤代谢的一些方面。
Aspects of 8-[C]Benzylaminopurine Metabolism in Phaseolus vulgaris.
机构信息
Institute of Pomology, University of Padova, 35100 Padova, Italy.
出版信息
Plant Physiol. 1979 Feb;63(2):298-300. doi: 10.1104/pp.63.2.298.
Phaseolus vulgaris L. plants were supplied through the root with [8-(14)C]benzylaminopurine ([(14)C]BA). Collections of root, apex, and leaves were made 8 and 48 hours after labeling; ethanolic extracts of tissues were purified and subjected to thin layer chromatography on silica gel and/or cellulose powder.From [(14)C]BA at least two different metabolites originate, the BA riboside and ribotide. The balance among [(14)C]BA, the riboside, and the ribotide changed in time. The [(14)C]BA riboside was detected as the only labeled compound in the xylem sap collected from the stem. The biological function of BA riboside and ribotide is discussed.
菜豆植株通过根部供给 [8-(14)C]苯氨基嘌呤 ([(14)C]BA)。标记后 8 小时和 48 小时收集根、顶芽和叶；组织的乙醇提取物经纯化后在硅胶和/或纤维素粉末上进行薄层层析。从 [(14)C]BA 至少产生两种不同的代谢物，即 BA 核苷和核苷。BA 与核苷和核苷之间的平衡随时间而变化。从茎中收集的木质部汁液中仅检测到 [(14)C]BA 核苷为标记化合物。BA 核苷和核苷的生物学功能正在讨论中。
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