Department of Botany, University of Indiana, Bloomington, Indiana 47401.
Plant Physiol. 1969 Mar;44(3):372-6. doi: 10.1104/pp.44.3.372.
A 6-(gamma,gamma-dimethylallylamino) purine-like compound was found in the culture medium of Rhizopogon roseolus, which had been shown earlier to synthesize zeatin. The role of 6-(gamma,gamma-dimethylallylamino) purine as a precursor of zeatin was studied. Rhizopogon was furnished with 6-(gamma,gamma-dimethylallylamino) purine-8-(14)C. Cochromatography, oxidation studies with potassium permanganate, and bromination indicated that labeled zeatin ribonucleoside was isolated from the medium. The fungus also incorporated labeled adenine, hypoxanthine, and 4-amino-5-imidazole carboxamide into zeatin ribonucleoside.
在曾被证明能够合成玉米素的红绒盖牛肝菌的培养液中发现了一种 6-(γ,γ-二甲基烯丙基氨基)嘌呤类似物。研究了 6-(γ,γ-二甲基烯丙基氨基)嘌呤作为玉米素前体的作用。向红绒盖牛肝菌提供 6-(γ,γ-二甲基烯丙基氨基)嘌呤-8-(14)C。共色谱分析、高锰酸钾氧化研究和溴化反应表明,标记的玉米素核苷从培养基中分离出来。该真菌还将标记的腺嘌呤、次黄嘌呤和 4-氨基-5-咪唑甲酰胺掺入到玉米素核苷中。