Davey J E, van Staden J
Department of Botany, University of Natal, Pietermaritzburg 3200, South Africa.
Plant Physiol. 1979 May;63(5):873-7. doi: 10.1104/pp.63.5.873.
Endogenous levels of cytokinin activity were examined in Lupinus albus L. seed at intervals of 2 weeks after anthesis using the soybean callus bioassay. High levels of cytokinin activity per gram seed material were present in the seeds at 2, 4, and 6 weeks after anthesis. The cytokinin activity per gram seed material was low at 8 and 10 weeks after anthesis. Cytokinin activity associated with each seed was greatest at 6 weeks after anthesis. The majority of the activity in the seeds at 4, 6, and 8 weeks after anthesis was in the endosperm. Cytokinin activity was also detected in the testas and embryos at 4, 6, 8, and 10 weeks, and the suspensors at 4 weeks. Column chromatography of extracts of the different seed fractions on Sephadex LH-20 indicated that the cytokinins present coeluted with zeatin, zeatin riboside, and the glucoside cytokinins. It is suggested that cytokinins are accumulated in the seeds and are stored in the endosperm mainly in the form of ribosides and glucosides of zeatin. The reduction in cytokinin activity in the seed coincides with the reduction in endosperm volume and embryo growth and suggests that these compounds are utilized during the course of seed maturation.
在羽扇豆花期过后，每隔2周使用大豆愈伤组织生物测定法检测白羽扇豆种子中细胞分裂素活性的内源水平。花期后2周、4周和6周时，每克种子材料中的细胞分裂素活性水平较高。花期后8周和10周时，每克种子材料中的细胞分裂素活性较低。与每粒种子相关的细胞分裂素活性在花期后6周时最高。花期后4周、6周和8周时，种子中的大部分活性存在于胚乳中。在花期后4周、6周、8周和10周时，种皮和胚中也检测到细胞分裂素活性，在花期后4周时，胚柄中也检测到细胞分裂素活性。不同种子组分提取物在Sephadex LH - 20上进行柱色谱分析表明，存在的细胞分裂素与玉米素、玉米素核苷和细胞分裂素糖苷共洗脱。研究表明，细胞分裂素在种子中积累，并主要以玉米素的核苷和糖苷形式储存在胚乳中。种子中细胞分裂素活性的降低与胚乳体积和胚生长的减少相一致，这表明这些化合物在种子成熟过程中被利用。
