Chemistry and Biology Research Institute, Research Branch, Agriculture Canada, Ottawa, Ontario K1A OC6, Canada.
Plant Physiol. 1976 Sep;58(3):253-6. doi: 10.1104/pp.58.3.253.
The elongation phase of growth of plant parts, as 1-cm segments excised from wheat coleoptiles here, is very simply recorded photometrically. One or more aligned segments submerged in aerated buffer pushed an Al foil shutter over a slit of light incident on a photodetector such as a solar or photronic cell, connected directly to a recorder, or a photomultiplier tube in a commercial photometer. Convenient kinetic records were obtained at 5 mm per min chart speed when one segment was combined with a 1-mm long slit, or more segments and longer slits, for which a 1-mm chart unit usually exceeded noise and was equivalent to 4 mum growth per cm coleoptile. In the presence of 10 cm of coleoptile segments no replenishment of solution was necessary during kinetic measurements of response in a 50-ml reservoir of IAA more concentrated than 30 nm.
植物部分生长的伸长阶段，如这里从小麦胚芽鞘中切下的 1 厘米段，可以非常简单地通过光度计进行记录。一个或多个对齐的段被浸没在充气缓冲液中，将铝箔快门推过一个狭缝光，该狭缝光入射到一个光电探测器上，例如一个太阳能或光电管，直接连接到一个记录仪上，或者连接到商业光度计中的光电倍增管上。当一个段与 1 毫米长的狭缝结合使用时，可以获得方便的动力学记录，其图表速度为每分钟 5 毫米，或者使用更多的段和更长的狭缝，对于后者，1 毫米图表单位通常超过噪声，相当于 4 微米每厘米胚芽鞘的生长。在存在 10 厘米长的胚芽鞘段的情况下，在 50 毫升 IAA 储液中进行动力学测量时，不需要补充溶液，该 IAA 储液的浓度比 30nm 更高。
