Section of Genetics, Development and Physiology, Division of Biological Sciences, Cornell University, Ithaca, New York 14850.
Plant Physiol. 1975 Mar;55(3):586-8. doi: 10.1104/pp.55.3.586.
Green pea (Pisum sativum L. var. Alaska) stem segments that were aged in buffer responded differently after aging depending on whether they were floating or submerged, or bubbled with air or N(2). Segments aged anaerobically for only 1 to 2 hours at 23 C responded to subsequent aerobic conditions by elongating more rapidly than aerobically aged sections. Longer periods of anaerobic treatment (up to 5 hours at 23 C) caused sections to exhibit an auxin-insensitive growth lag and reversible shrinkage. The shrinkage accelerated upon return to aerobic conditions but reversed after 1 to 2 hours. Green pea stem segments therefore require vigorous aeration during aging and growth measurements.
绿豌豆(Pisum sativum L. var. Alaska)茎段在缓冲液中老化时,根据其是漂浮还是浸没,或是否通空气或 N(2),反应会有所不同。在 23°C 下仅厌氧老化 1 至 2 小时的茎段在随后的需氧条件下的伸长速度比有氧老化的部分更快。较长时间的厌氧处理(在 23°C 下长达 5 小时)会导致部分表现出对生长素不敏感的生长滞后和可逆转的收缩。在返回需氧条件下,收缩会加速,但在 1 至 2 小时后会逆转。因此,在老化和生长测量期间,绿豌豆茎段需要强烈通气。