蓝光细菌鱼腥藻细胞质中 pH 值的光依赖性变化，由质子进入类囊体空间引起。
pH Changes in the Cytoplasm of the Blue-Green Alga Anacystis nidulans Caused by Light-dependent Proton Flux into the Thylakoid Space.
机构信息
Limnologisches Institut der Osterreichischen Akademie der Wissenschaften.
出版信息
Plant Physiol. 1976 Dec;58(6):717-8. doi: 10.1104/pp.58.6.717.
Abstract
The pH in the cytoplasmic and thylakoid spaces of the blue-green alga, Anacystis nidulans, has been determined in the light and in the dark by uptake of 5,5-dimethyloxazolidine-2,4-dione and methylamine into the sucrose-impermeable (3)H-H(2)O space, as measured by silicon layer filtering centrifugation.Illumination causes an alkalinization in the cytoplasm which is accompanied by an acidification in the thylakoid space, reflecting light-dependent proton transport across the thylakoid membrane. Under light conditions, a pH gradient of approximately 2.8 between the cytoplasmic and thylakoid spaces has been measured that can be abolished almost completely by addition of the uncoupler, 3-chlorocarbonyl cyanide phenylhydrazone. The pH in the cytoplasm is independent of the pH in the medium.
摘要
蓝绿藻鱼腥藻的细胞质和类囊体空间的 pH 值，通过 5,5-二甲基恶唑烷-2,4-二酮和甲胺进入蔗糖不可渗透的（3）H-H2O 空间来测定，通过硅层过滤离心测量。光照引起细胞质的碱化，同时类囊体空间酸化，反映了光依赖的质子穿过类囊体膜的运输。在光照条件下，测量到细胞质和类囊体空间之间约 2.8 的 pH 梯度，该梯度可以通过加入解偶联剂 3-氯甲酰基氰基苯腙几乎完全消除。细胞质中的 pH 值与培养基中的 pH 值无关。
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本文引用的文献
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The inner membrane of the chloroplast envelope as the site of specific metabolite transport.叶绿体被膜内膜作为特定代谢物运输的场所。
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Alkalization of the chloroplast stroma caused by light-dependent proton flux into the thylakoid space.光依赖质子通量进入类囊体腔导致叶绿体基质碱化。
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