Institut für Botanik und Pharmazeutische Biologie mit Botanischem Garten, Mittlerer Dallenbergweg 64, D-8700, Würzburg, Germany.
Planta. 1980 Jul;149(2):138-43. doi: 10.1007/BF00380874.
Investigations of the energy-dependent accumulation of orthophosphate by the blue-green alga Anacystis nidulans have established: 1. The transport through the cell membrane is the rate-limiting step in the incorporation of phosphate.-2. This transport is facilitated by a "carrier" that can be activated by Ca(2+) and Mg(2+) and inhibited by EDTA.-3. The activation of the carrier in the light is associated with changes of the cytoplasmic Mg(2+) content.-4. Intracellular phosphate is shown to be present in bound form.-5. The energy-dependent accumulation of orthophosphate within the cell depends strictly on the cytoplasmic pH and not on the energy conversion at the thylakoid membrane which is responsible for the energy supply. The cytoplasmic pH is different in the light, in the dark, and in the presence of the uncoupler carbonyl cyanide m-chlorophenylhydrazone (CCCP). Orthophosphate accumulation can most readily be explained in terms of a pH dependent precipitation into a complex with bivalent cations rather than by an active transport against a concentration gradient.
对蓝绿藻鱼腥藻中 orthophosphate 依赖能量积累的研究已经确定：1. 通过细胞膜的运输是磷掺入的限速步骤。2. 这种运输由一种“载体”促进，该载体可以被 Ca(2+)和 Mg(2+)激活，被 EDTA 抑制。3. 光中载体的激活与细胞质 Mg(2+)含量的变化有关。4. 细胞内磷酸盐以结合形式存在。5. 细胞内 orthophosphate 的能量依赖积累严格依赖于细胞质 pH，而不依赖于负责能量供应的类囊体膜的能量转换。细胞质 pH 在光、暗和存在解偶联剂羰基氰化物 m-氯苯腙 (CCCP) 时是不同的。orthophosphate 积累最容易用依赖 pH 的沉淀解释为与二价阳离子形成复合物，而不是逆浓度梯度的主动运输。
