Department of Biology, Brandeis University, Waltham, Massachusetts 02154.
Plant Physiol. 1977 Apr;59(4):767-70. doi: 10.1104/pp.59.4.767.
Red light-induced, far red light-reversible increase in etiolated bean (Phaseolus vulgaris, var. Asgrow Valentine) leaf polyribosomes was shown to be sensitive to actinomycin D, cycloheximide, and rifampicin inhibition. Actinomycin prevented response to red light if administered simultaneously with a 10-minute illumination, had no immediate effect if given 2 hours after illumination, but was again rapidly inhibitory at 4 and 6 hours. The effects of actinomycin and far red light were more than additive.Cycloheximide consistently inhibited polysome formation within 1 hour at all times tested.Formation of both cytoplasmic and organelle polysomes was depressed by rifampicin, an antibiotic whose action is primarily on RNA synthesis in organelles.The results are interpreted to show that light-triggered polysome formation exhibits a changing need for RNA synthesis and an obligate dependence on protein synthesis during the 1st hours following brief illumination.
红光诱导的、远红光可逆的黄化菜豆（Phaseolus vulgaris，var. Asgrow Valentine）叶片多核糖体的增加，对放线菌素 D、环己酰亚胺和利福平抑制敏感。如果在 10 分钟的光照同时给予放线菌素 D，它会阻止对红光的反应；如果在光照后 2 小时给予，它没有立即作用，但在 4 小时和 6 小时再次迅速抑制。放线菌素 D 和远红光的作用具有加性。环己酰亚胺在所有测试时间内都始终在 1 小时内一致抑制多核糖体的形成。利福平抑制细胞质和细胞器多核糖体的形成，利福平是一种主要作用于细胞器中 RNA 合成的抗生素。结果表明，光触发的多核糖体形成在短暂光照后的头 1 小时内表现出对 RNA 合成的不断需求和对蛋白质合成的必需依赖性。
