温度诱导的来自冷敏感和抗冷植物的叶绿体希尔活性变化。
Temperature-induced Changes in Hill Activity of Chloroplasts Isolated from Chilling-sensitive and Chilling-resistant Plants.
作者信息
Nolan W G, Smillie R M
机构信息
Plant Physiology Unit, CSIRO Division of Food Research and School of Biological Sciences, Macquarie University, North Ryde 2113, Sydney, Australia.
出版信息
Plant Physiol. 1977 Jun;59(6):1141-5. doi: 10.1104/pp.59.6.1141.
The effect of temperature on Hill activity has been compared in chilling-sensitive and chilling-resistant plants. The Arrhenius activation energy (Ea) for the photoreduction of 2,6-dichlorophenolindophenol by chloroplasts isolated from two chilling-sensitive plants, mung bean (Vigna radiata L. var. Mungo) and maize (Zea mays L. cv. PX 616), increased at low temperatures, below 17 C for mung bean and below 11 C for maize. However, the Ea for this reaction in pea (Pisum sativum L. cv. Massay Gem), a chilling-resistant plant, likewise increased at temperatures below 14 C. A second change in Ea occurred at higher temperatures. The Ea decreased above about 28 C for mung bean, 30 C for maize, and 25 C for pea. At temperatures approaching 40 C, thermal inactivation of Hill activity occurred. These results, when taken together with previous results obtained with the chilling-resistant plant barley, indicate that chloroplasts from both chilling-sensitive and chilling-resistant plants can undergo a change in chloroplast membrane activity at low temperatures above freezing and that the presence of such a change in chloroplast membranes is not necessarily correlated with chilling sensitivity.
已对冷敏感植物和抗冷植物中温度对希尔反应活性的影响进行了比较。从两种冷敏感植物绿豆（Vigna radiata L. var. Mungo）和玉米（Zea mays L. cv. PX 616）分离得到的叶绿体对2,6 - 二氯酚靛酚进行光还原的阿累尼乌斯活化能（Ea）在低温时增加，绿豆在17℃以下、玉米在11℃以下时Ea增加。然而，抗冷植物豌豆（Pisum sativum L. cv. Massay Gem）中该反应的Ea在14℃以下时同样增加。在较高温度下Ea发生了第二次变化。绿豆在约28℃以上、玉米在30℃以上、豌豆在25℃以上时Ea下降。在接近40℃的温度下，希尔反应活性发生热失活。这些结果与之前用抗冷植物大麦得到的结果一起表明，冷敏感植物和抗冷植物的叶绿体在高于冰点的低温下都可能发生叶绿体膜活性的变化，并且叶绿体膜中存在这种变化不一定与冷敏感性相关。
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