Department of Horticulture, Michigan State University, East Lansing, Michigan, USA.
Theor Appl Genet. 1981 Sep;59(5):265-8. doi: 10.1007/BF00264977.
One to five percent of Lycopersicon peruvianum (L.) Mill. leaf mesophyll protoplasts undergo cell division and concomitant organization to form embryogenic-like structures when cultured in Murashige and Skoog medium (1962) containing 3% sucrose, 9% mannitol, 1.0 mg/l kinetin (K) and 1.0 mg/l naphthalene acetic acid (NAA) at pH 5.6-5.8 (medium A). These embryogenic structures, after passing through developmental stages similar to those observed in zygotic embryogeny, are capable of forming shoots on hormone-free medium A. In medium B, wherein 0.5 mg/l of 2,4-dichlorophenoxyacetic (2,4-D) replaced the hormones (K and NAA), embryogenic structures did not develop. However, callus originating in medium B retained morphogenetic capacity as was evidenced by subsequent shoot regeneration when they were transferred to medium A with K and NAA replaced by 1.0 mg/l zeatin (Z). The potential value of incorporating this regeneration trait into Lycopersicon species and cultivated lines for use in tissue culture programs is discussed.
当在含有 3%蔗糖、9%甘露醇、1.0mg/L 激动素（K）和 1.0mg/L 萘乙酸（NAA）、pH5.6-5.8（培养基 A）的 Murashige 和 Skoog 培养基中培养时，秘鲁番茄（Lycopersicon peruvianum (L.) Mill.）叶片叶肉原生质体的 1%至 5%会经历细胞分裂和伴随的组织化，形成胚胎发生样结构。这些胚胎发生结构在经历类似于合子胚胎发生中观察到的发育阶段后，能够在无激素的培养基 A 上形成芽。在培养基 B 中，用 0.5mg/L 2,4-二氯苯氧乙酸（2,4-D）替代激素（K 和 NAA），胚胎发生结构不会发育。然而，源自培养基 B 的愈伤组织保留了形态发生能力，这可以通过随后将其转移到用 K 和 NAA 替代为 1.0mg/L 玉米素（Z）的培养基 A 上进行芽再生来证明。讨论了将这种再生特性纳入番茄属物种和栽培系以用于组织培养计划的潜在价值。
