来自自由生活型和共生型豌豆根瘤菌（PRE菌株）的DNA比较。

A comparison of DNA from free living and endosymbiotic Rhizobium leguminosarum (strain PRE).

Reijnders L, Visser L, Aalbers A M, Van Kammen A, Houwers A

Biochim Biophys Acta. 1975 Dec 4;414(2):206-16. doi: 10.1016/0005-2787(75)90224-5.

Abstract

Bacteroids of Rhizobium leguminosarum (strain PRE) purified from root nodules of Pisum sativum (var. 'Rondo') by the standard procedure of differential centrifugation contained considerable contamination of mitochondrial material. This could be removed by incubation of the bacteroid preparation with 1 M KCl/1% deoxycholate. 2. The DNA content of bacteroid cells of R. leguminosarum was found to have increased about three fold in comparison with the DNA content of free living R. leguminosarum bacteria. 3. No significant difference in DNA composition of free living R. leguminosarum bacteria and bacteroids could be detected by CsCl equilibrium centrifugation, RNA - DNA hybridization and DNA - DNA reassociation studies.

摘要

通过差速离心的标准程序从豌豆（品种‘隆多’）根瘤中纯化得到的豌豆根瘤菌（菌株PRE）类菌体含有大量线粒体物质污染。通过将类菌体制剂与1 M KCl/1%脱氧胆酸盐一起孵育可去除这种污染。2. 发现豌豆根瘤菌类菌体细胞的DNA含量相较于自由生活的豌豆根瘤菌细菌的DNA含量增加了约三倍。3. 通过CsCl平衡离心、RNA - DNA杂交和DNA - DNA重缔合研究，未检测到自由生活的豌豆根瘤菌细菌和类菌体在DNA组成上有显著差异。