Dogras C C, Dilley D R, Herner R C
Department of Horticulture, Michigan State University, East Lansing, Michigan 48824.
Plant Physiol. 1977 Dec;60(6):897-902. doi: 10.1104/pp.60.6.897.
The proportion of labeled (14)C-glycerol incorporated into phospholipids and the fatty acid composition of three phospholipids in germinating seeds and seedlings of chilling-sensitive lima beans (Phaseolus lunatus L.) and chilling-resistant broad beans (Vicia faba L.) and peas (Pisum sativum L.) at 10 and 25 C were determined. During the imbibition of seeds (first 24 hours), lima beans were sensitive to chilling injury at 10 C and a higher proportion of label was incorporated into phosphatidylethanolamine and phosphatidylglycerol than in broad beans and peas. Broad beans and peas incorporated a higher proportion of label into phosphatidylcholine. The oleic acid content of phosphatidylcholine was higher and linolenic acid content was lower in peas and broad beans than in lima beans at 10 and 25 C. The unsaturated to saturated fatty acid ratio was much higher for the chilling-resistant seeds than for the chilling-sensitive ones. In the seedling stage, the proportion of label incorporated into the four major phospholipids was similar in the three species regardless of temperature treatment. The fatty acid content of the phospholipids examined was not different in the three species in the seedling stage.
测定了在10℃和25℃下，冷敏感的利马豆（菜豆属）、抗寒的蚕豆（蚕豆属）和豌豆（豌豆属）萌发种子及幼苗中，标记的（14）C-甘油掺入磷脂的比例以及三种磷脂的脂肪酸组成。在种子吸胀期间（最初24小时），利马豆在10℃下对冷害敏感，与蚕豆和豌豆相比，有更高比例的标记物掺入磷脂酰乙醇胺和磷脂酰甘油中。蚕豆和豌豆将更高比例的标记物掺入磷脂酰胆碱中。在10℃和25℃下，豌豆和蚕豆中磷脂酰胆碱的油酸含量较高，亚麻酸含量较低。抗寒种子的不饱和脂肪酸与饱和脂肪酸的比例远高于冷敏感种子。在幼苗期，无论温度处理如何，三种植物中掺入四种主要磷脂的标记物比例相似。在幼苗期，所检测的三种植物中磷脂的脂肪酸含量没有差异。
