Davies D D, Humphrey T J
School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom.
Plant Physiol. 1978 Jan;61(1):54-8. doi: 10.1104/pp.61.1.54.
Methods of measuring amino acid recycling in Lemna minor are described. The extent to which the recycling of individual amino acids may underestimate protein turnover has been measured for a number of amino acids. The methods have been used to study the relationship between protein turnover and amino acid recycling during nitrogen starvation. It is concluded that following the removal of nitrate from the environment, protein turnover is enhanced, the partitioning of amino acids between protein synthesis and amino acid metabolism is relatively constant, but the total amount of amino acids recycling is increased.
本文描述了测量浮萍中氨基酸循环的方法。对于多种氨基酸,已测定了单个氨基酸的循环在多大程度上可能低估蛋白质周转。这些方法已用于研究氮饥饿期间蛋白质周转与氨基酸循环之间的关系。得出的结论是,从环境中去除硝酸盐后,蛋白质周转增强,氨基酸在蛋白质合成和氨基酸代谢之间的分配相对恒定,但氨基酸循环的总量增加。