Botanical Institute of Johann Wolfgang Goethe-University, Siesmayerstrasse 70, D-60323, Frankfurt/Main, Germany.
Environ Sci Pollut Res Int. 1997;4(1):37-45. doi: 10.1007/BF02986263.
Practical considerations, characteristic physiological features, as well as the availability of hybrids with different resistances to air pollutants play an important role in the decision to use poplar cuttings as experimental plants for impact studies.This paper demonstrates the effect of the hybridization of two poplar clones on the resistance to different air pollutants and, additionally, presents a new concept of bioindication involving these two clones with different degrees of resistance to SO(2) and O(3).This concept, which is based on the results of fumigation experiments conducted in open-top chambers, uses six-week-old vital poplar cuttings as bioindicators. Both the anabolic defense reactions of younger leaves and the catabolic stress reactions of older leaves are examined. The measurement results obtained from cv. Loenen and cv. Rochester clones which differ in their sensitivities to individual pollutants, are examined to determine the specific relationships. The results can be verified by measuring the premature leaf losses of a third clone, cv. Unal, which reacts to both SO(2) and O(3) with highly pronounced leaf loss. To prevent false interpretations, it is necessary to standardize the plant material as far as possible by choosing clones of a defined age and number of leaves, and by selecting reproducible cultivation and exposure conditions. The impact criteria are methodologically simple and therefore suitable for routine examinations.
实用考虑因素、特征生理特征以及具有不同抗空气污染物能力的杂种的可用性在决定使用杨树插条作为影响研究的实验植物方面起着重要作用。本文展示了两个杨树无性系杂交对不同空气污染物抗性的影响,此外,还提出了一个涉及这两个具有不同 SO2 和 O3 抗性的无性系的生物指示新概念。该概念基于在开顶室中进行的熏气实验的结果,使用六周龄的有生命力的杨树插条作为生物指示剂。检查年轻叶片的合成防御反应和较老叶片的分解代谢应激反应。检查了对个别污染物敏感性不同的 cv. Loenen 和 cv. Rochester 无性系的测量结果,以确定特定关系。通过测量对 SO2 和 O3 均有明显叶片损失的第三个无性系 cv. Unal 的过早叶片损失,可以验证结果。为了防止错误解释,有必要通过选择具有明确定义的年龄和叶片数量的无性系,以及选择可重复的栽培和暴露条件,尽可能使植物材料标准化。影响标准在方法上很简单,因此适合常规检查。