Department of Crop Sciences, Experiment Station, Hawaiian Sugar Planters' Association, Aiea, Hawaii 96701.
Plant Physiol. 1978 Apr;61(4):544-8. doi: 10.1104/pp.61.4.544.
Although d-galactose is normally toxic to sugarcane (Saccharum sp.) cells, a cell line that grows on 100 mm galactose has been propagated. Nonadapted cells in a medium containing galactose instead of sucrose accumulate UDP-galactose; these cells also have much lower UDP-galactose 4-epimerase (EC 5.1.3.2) activity than do adapted cells. This enzyme may determine whether or not galactose will cause toxicity symptoms to develop. The growth rate of galactose-adapted cells is similar to most cell lines on several other carbohydrates. The galactose-adapted cells are also similar to sucrose stock cells in cell wall composition and sugar phosphate concentrations, but, like the nonadapted cells, accumulate free galactose.
尽管半乳糖通常对甘蔗(Saccharum sp.)细胞有毒性,但已经繁殖了一种在 100mm 半乳糖上生长的细胞系。在含有半乳糖而不是蔗糖的培养基中的非适应细胞积累 UDP-半乳糖;与适应细胞相比,这些细胞的 UDP-半乳糖 4-差向异构酶(EC 5.1.3.2)活性也低得多。该酶可能决定半乳糖是否会导致毒性症状的发展。适应半乳糖的细胞的生长速率与其他几种碳水化合物上的大多数细胞系相似。适应半乳糖的细胞在细胞壁组成和糖磷酸浓度方面也与蔗糖库存细胞相似,但与非适应细胞一样,它们也会积累游离半乳糖。