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在悬浮培养中适应半乳糖的甘蔗细胞系的特征。

Characteristics of a Galactose-adapted Sugarcane Cell Line Grown in Suspension Culture.

机构信息

Department of Crop Sciences, Experiment Station, Hawaiian Sugar Planters' Association, Aiea, Hawaii 96701.

出版信息

Plant Physiol. 1978 Apr;61(4):544-8. doi: 10.1104/pp.61.4.544.

DOI:10.1104/pp.61.4.544
PMID:16660333
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1091914/
Abstract

Although d-galactose is normally toxic to sugarcane (Saccharum sp.) cells, a cell line that grows on 100 mm galactose has been propagated. Nonadapted cells in a medium containing galactose instead of sucrose accumulate UDP-galactose; these cells also have much lower UDP-galactose 4-epimerase (EC 5.1.3.2) activity than do adapted cells. This enzyme may determine whether or not galactose will cause toxicity symptoms to develop. The growth rate of galactose-adapted cells is similar to most cell lines on several other carbohydrates. The galactose-adapted cells are also similar to sucrose stock cells in cell wall composition and sugar phosphate concentrations, but, like the nonadapted cells, accumulate free galactose.

摘要

尽管半乳糖通常对甘蔗（Saccharum sp.）细胞有毒性，但已经繁殖了一种在 100mm 半乳糖上生长的细胞系。在含有半乳糖而不是蔗糖的培养基中的非适应细胞积累 UDP-半乳糖；与适应细胞相比，这些细胞的 UDP-半乳糖 4-差向异构酶（EC 5.1.3.2）活性也低得多。该酶可能决定半乳糖是否会导致毒性症状的发展。适应半乳糖的细胞的生长速率与其他几种碳水化合物上的大多数细胞系相似。适应半乳糖的细胞在细胞壁组成和糖磷酸浓度方面也与蔗糖库存细胞相似，但与非适应细胞一样，它们也会积累游离半乳糖。

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Planta. 1981 Dec;153(4):370-5. doi: 10.1007/BF00384256.
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本文引用的文献

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Influence of carbohydrates on quantitative aspects of growth and embryo formation in wild carrot suspension cultures.碳水化合物对野生胡萝卜悬浮培养物生长和胚胎形成定量方面的影响。
Plant Physiol. 1977 Jan;59(1):81-5. doi: 10.1104/pp.59.1.81.
2
The Structure of Plant Cell Walls: VI. A Survey of the Walls of Suspension-cultured Monocots.植物细胞壁的结构：VI. 悬浮培养单子叶植物细胞壁综述
Plant Physiol. 1974 Jul;54(1):109-15. doi: 10.1104/pp.54.1.109.
3
Membrane transport of sugars in cell suspensions of sugarcane: I. Evidence for sites and specificity.甘蔗细胞悬浮液中糖的膜转运：I. 位点和特异性的证据。
Plant Physiol. 1972 Feb;49(2):177-82. doi: 10.1104/pp.49.2.177.
4
Growth Inhibition and Metabolite Pool Levels in Plant Tissues Fed d-Glucosamine and d-Galactose.植物组织喂养 d-葡萄糖胺和 d-半乳糖时的生长抑制和代谢物池水平。
Plant Physiol. 1971 Jul;48(1):36-42. doi: 10.1104/pp.48.1.36.
5
Nucleoside Diphosphate-sugar 4-Epimerases I. Uridine Diphosphate Glucose 4-Epimerase of Wheat Germ.核苷酸二磷酸糖 4-差向异构酶 I. 麦胚尿苷二磷酸葡萄糖 4-差向异构酶。
Plant Physiol. 1969 Apr;44(4):599-604. doi: 10.1104/pp.44.4.599.
6
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8
A mammalian uridinediphosphate galactose pyrophosphorylase.一种哺乳动物尿苷二磷酸半乳糖焦磷酸化酶。
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9
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10
Metabolism of galactose in Canna leaves and wheat seedlings.美人蕉叶片和小麦幼苗中半乳糖的代谢
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