Department of Agricultural Biochemistry, School of Agriculture, University of Edinburgh, West Mains Road, Edinburgh EH9 3JG, Scotland.
Plant Physiol. 1978 Apr;61(4):570-4. doi: 10.1104/pp.61.4.570.
Changes in the activity of a number of enzymes concerned with amino acid synthesis and metabolism were recorded for the endosperm, testa pericarp, and embryo of developing barley (Hordeum distichum L.) grains. Both glutamate-pyruvate transaminase and glutamate-oxaloacetate transaminase activities were present in all tissues and at all ages examined. Glutamate dehydrogenase activity was largely confined to endosperm while glutamine synthetase activity was mainly in the testa pericarp.Ammonium ion concentration was maximal in endosperm by 20 days after anthesis. Glutamate concentration varied in endosperm and was in the range of 3.5 to 8.5 mm between 20 and 45 days after anthesis. Significant levels of ammonium ion and glutamate were also present in the testa pericarp over the major part of the developmental period.
研究了发育中的大麦（Hordeum distichum L.）籽粒胚乳、种皮和胚中与氨基酸合成和代谢有关的一些酶的活性变化。在所有检查的组织和时期都存在谷氨酸-丙酮酸转氨酶和谷氨酸-草酰乙酸转氨酶活性。谷氨酸脱氢酶活性主要局限于胚乳，而谷氨酰胺合成酶活性主要存在于种皮中。在授粉后 20 天，胚乳中的铵离子浓度达到最大值。谷氨酸浓度在胚乳中变化，授粉后 20 至 45 天期间，谷氨酸浓度在 3.5 至 8.5mm 之间。在发育的大部分时间里，种皮中也存在显著水平的铵离子和谷氨酸。
