Pueppke S G, Bauer W D
Charles F. Kettering Research Laboratory, 150 East South College Street, Yellow Springs, Ohio 45387.
Plant Physiol. 1978 May;61(5):779-84. doi: 10.1104/pp.61.5.779.
Three different assay procedures have been used to quantitate the levels of soybean (Glycine max [L.] Merr.) lectin in various tissues of soybean plants. The assays used were a standard hemagglutination assay, a radioimmunoassay, and an isotope dilution assay. Most of the lectin in seeds was found in the cotyledons, but lectin was also detected in the embryo axis and the seed coat. Soybean lectin was present in all of the tissues of young seedlings, but decreased as the plants matured and was not detectable in plants older than 2 to 3 weeks. Soybean lectin isolated from seeds of several soybean varieties were identical when compared by several methods.
已采用三种不同的测定方法来定量大豆植株不同组织中大豆（Glycine max [L.] Merr.）凝集素的水平。所使用的测定方法为标准血凝测定法、放射免疫测定法和同位素稀释测定法。种子中的大部分凝集素存在于子叶中，但在胚轴和种皮中也检测到了凝集素。大豆凝集素存在于幼苗的所有组织中，但随着植株成熟而减少，在2至3周龄以上的植株中无法检测到。通过几种方法比较时，从几个大豆品种的种子中分离出的大豆凝集素是相同的。
