Bhuvaneswari T V, Bauer W D
Charles F. Kettering Research Laboratory, 150 East South College Street, Yellow Springs, Ohio 45387.
Plant Physiol. 1978 Jul;62(1):71-4. doi: 10.1104/pp.62.1.71.
The influence of rhizosphere/rhizoplane culture conditions on the ability of various rhizobia to bind soybean seed lectin (SBL) was examined. Eleven strains of the soybean symbiont, Rhizobium japonicum, and six strains of various heterologous Rhizobium species were cultured in root exudate of soybean (Glycine max [L.] Merr.) and in association with roots of soybean seedlings which were growing either hydroponically or in montmorillonite clay soil amendment (Turface). All 11 of the R. japonicum strains developed biochemically specific receptors for the lectin when cultured under these conditions, whereas six of the 11 did not develop such receptors when cultured in synthetic salts medium. Two cowpea strains also developed receptors for SBL. The other four heterologous strains of rhizobia gave no evidence of biochemically specific SBL binding in either synthetic salts media or rhizosphere/rhizoplane cultures. These results demonstrate that the environment provided by plant roots is an important factor in the development of specific lectin receptors on the cell surface of R. japonicum.
研究了根际/根面培养条件对各种根瘤菌结合大豆种子凝集素（SBL）能力的影响。将11株大豆共生菌日本根瘤菌和6株不同的异源根瘤菌菌株，在大豆（Glycine max [L.] Merr.）根分泌物中培养，并与水培或在蒙脱石粘土改良剂（Turface）中生长的大豆幼苗根系共生培养。在这些条件下培养时，所有11株日本根瘤菌菌株都产生了针对该凝集素的生化特异性受体，而在合成盐培养基中培养时，11株中有6株没有产生这样的受体。两株豇豆根瘤菌菌株也产生了SBL受体。其他4株异源根瘤菌菌株在合成盐培养基或根际/根面培养中均未显示出生化特异性SBL结合的证据。这些结果表明，植物根系提供的环境是日本根瘤菌细胞表面特异性凝集素受体形成的一个重要因素。
