Department of Botany, University of Georgia, 30602, Athens, GA, USA.
Planta. 1984 Sep;162(1):55-61. doi: 10.1007/BF00397421.
Radioimmuno-and enzyme-linked immunosorbent assays show that a substantial amount of wheat germ agglutinin(WGA)-like protein is present at the base of the shoot and in the roots of adult wheat (Triticum aestivum L.) plants. The protein can be purified by hapten-and antibody-mediated affinity procedures. It forms an arc of identity with the embryo lectin upon Ouchterlony double-diffusion and is an active lectin that agglutinates trypsinized erythrocytes in an N-acetylglucosamine-and chitin-inhibitable manner. Reduced and carboxyamidated protein comigrates with the 18-kdalton subunits of embryo lectin on sodium dodecyl sulfate-polyacrylamide gels. Invivo labeling of 9-d-old, hydroponically grown plants with (35)S-labeled sulfate demonstrates that at least some of the WGA-like protein is synthesized de novo. Immunocytochemistry with rabbit anti-WGA and colloidal-gold-conjugated second antibody shows that cross-reactive protein is present at the tips of new adventitious roots. In reactive cells, the lectin is localized near the inner surface of the vacuole membrane. Wheat plants contain up to 100 ng of WGA-like protein after the first week of growth, but the level fluctuates thereafter. Since most of the lectin is present at the base of the shoot and much less is found in older roots, these fluctuations may be the consequence of changes in the initiation of new advantitious roots.
放射免疫和酶联免疫吸附试验表明，大量的麦胚凝集素（WGA）样蛋白存在于小麦（Triticum aestivum L.）植株的芽基和根部。该蛋白可通过半抗原和抗体介导的亲和程序进行纯化。它与胚胎凝集素在奥奇特里隆双扩散中形成一个弧形的同一性，是一种活性凝集素，以 N-乙酰葡萄糖胺和几丁质抑制的方式凝集胰蛋白酶化的红细胞。还原和羧基酰胺化的蛋白质在十二烷基硫酸钠-聚丙烯酰胺凝胶上与胚胎凝集素的 18kDa 亚基共迁移。用（35）S 标记的硫酸盐对 9 天大的水培植物进行体内标记表明，至少有一些 WGA 样蛋白是从头合成的。用兔抗 WGA 和胶体金缀合的第二抗体进行免疫细胞化学染色表明，交叉反应蛋白存在于新不定根的尖端。在反应性细胞中，凝集素定位于液泡膜的内表面附近。小麦植株在生长第一周后含有多达 100ng 的 WGA 样蛋白，但此后水平波动。由于大部分凝集素存在于芽基，而在较老的根中发现的凝集素较少，这些波动可能是新不定根起始变化的结果。
