利用核磁共振测定冻融损伤后冬小麦细胞的膜通透性变化
Changes in Membrane Permeability of Winter Wheat Cells following Freeze-Thaw Injury as Determined by Nuclear Magnetic Resonance.
作者信息
Chen P M, Gusta L V
机构信息
Crop Development Center, University of Saskatchewan, Saskatoon.
出版信息
Plant Physiol. 1978 Jun;61(6):878-82. doi: 10.1104/pp.61.6.878.
Nuclear magnetic resonance (NMR) relaxation times were studied in acclimated and nonacclimated Kharkov winter wheat (Triticum aestivum L.) crowns and acclimated cell aggregates to determine if membrane permeability was altered by freezing. The NMR water signal decay consisted of two exponential components: a short one arising from extracellular water, and a long one arising from intracellular water. A slow freezethaw treatment of nonacclimated and 1-week acclimated crowns decreased the long relaxation time, suggesting membrane injury. Similar results were obtained for nonacclimated and acclimated crowns killed directly in liquid N(2).A significant increase in plasma membrane permeability to Mn(2+) was observed in acclimated freeze-killed crowns and cell aggregates. Freezing injury to plant tissue appears to be a membrane-related phenomenon, but more extensive injury occurs to nonacclimated and acclimated tissue with a high water content (cell aggregates) compared to acclimated tissue with a low water content (crowns).
对经过驯化和未经驯化的哈尔科夫冬小麦（Triticum aestivum L.）冠部以及经过驯化的细胞聚集体进行了核磁共振（NMR）弛豫时间研究，以确定冷冻是否会改变膜通透性。核磁共振水信号衰减由两个指数成分组成：一个短的成分来自细胞外水，一个长的成分来自细胞内水。对未经驯化和经过1周驯化的冠部进行缓慢冻融处理会降低长弛豫时间，表明膜受到损伤。对于直接在液氮中杀死的未经驯化和经过驯化的冠部，也得到了类似结果。在经过驯化的冻害冠部和细胞聚集体中，观察到质膜对Mn(2+)的通透性显著增加。植物组织的冻害似乎是一种与膜相关的现象，但与含水量低的驯化组织（冠部）相比，含水量高的未经驯化和经过驯化的组织（细胞聚集体）会受到更广泛的损伤。
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