Department of Biochemistry, University of Nevada, Reno, Nevada 89507.
Plant Physiol. 1978 Jun;61(6):949-52. doi: 10.1104/pp.61.6.949.
A wide variety of plants have been assayed to determine if they contain three isozymes of chorismate mutase (EC 5.4.99.5) as does alfalfa (Medicago sativa L.) or two isozymes, as does mung bean (Phaseolus aureus). The isozymes were separated by disc electrophoresis. All anthophyta with the exception of some closely related Leguminosae contained three isozymes of chorismate mutase. The one coniferophyta (a pine), and pterophyta (a fern) and one microphyllophyta (a Selaginella) assayed contained two isozymes of chorismate mutase. All plants assayed contained measurable chorismate mutase levels and at least two isozymes of chorismate mutase.
已对多种植物进行了测定,以确定它们是否含有与紫花苜蓿(Medicago sativa L.)相同的三种分支酸变位酶(EC 5.4.99.5)同工酶,或是否含有与绿豆(Phaseolus aureus)相同的两种同工酶。同工酶通过圆盘电泳进行分离。除了一些密切相关的豆科植物外,所有的种子植物都含有三种分支酸变位酶同工酶。所测试的一种针叶树(松树)、一种蕨类植物和一种小型叶植物(卷柏)含有两种分支酸变位酶同工酶。所有测试的植物都含有可测量的分支酸变位酶水平和至少两种分支酸变位酶同工酶。