Department of Biochemistry and Biophysics, University of California, Davis, California 95616.
Plant Physiol. 1986 Jul;81(3):717-22. doi: 10.1104/pp.81.3.717.
The isoenzyme pattern of chorismate mutase (EC 5.4.99.5) was examined by diethylaminoethyl-cellulose chromatography in a wide variety of plants. All plants contained a regulated form of chorismate mutase (CM-1), and most contained an additional, unregulated form (CM-2). The regulatory properties of CM-1 differed significantly between plants. Antisera prepared against CM-1 and CM-2 from Sorghum bicolor were used to test immunological cross reaction of chorismate mutases from other plants. There was a high degree of similarity between chorismate mutase isoenzymes from Sorghum bicolor and Zea mays and some with Hordeum vulgare, but all other species studied were antigenically distinct from sorghum. No homology between the structure of CM-1 and CM-2 was detected within any species.
在广泛的植物中,通过二乙基氨基乙基纤维素层析法检查了分支酸变位酶(EC 5.4.99.5)的同工酶模式。所有植物都含有调节形式的分支酸变位酶(CM-1),大多数植物还含有另一种未调节的形式(CM-2)。CM-1 的调节特性在植物之间有很大的差异。针对高粱 CM-1 和 CM-2 制备的抗血清用于检测来自其他植物的分支酸变位酶的免疫交叉反应。高粱和玉米的分支酸变位酶同工酶之间具有高度的相似性,并且与大麦的一些同工酶也具有相似性,但所有其他研究的物种与高粱在抗原上都是不同的。在任何物种中都没有检测到 CM-1 和 CM-2 之间的结构同源性。