Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019.
Plant Physiol. 1984 May;75(1):251-2. doi: 10.1104/pp.75.1.251.
We have shown in a previous publication (Gupta, Beevers 1983 J Exp Bot 34: 1455-1462) that the level of extractable nitrite reductase activity in pea (Pisum sativum cv Burpeeana) leaves is subject to environmental perturbations. In the current study, we have used rocket immunoelectrophoresis to quantitate the level of nitrite reductase protein in extracts from pea plants subjected to various environmental treatments. The level of nitrite reductase cross-reacting material closely followed the level of assayable nitrite reductase activity. The environmental conditions which enhanced the level of extractable nitrite reductase activity resulted in an increased level of nitrite reductase cross-reacting material in the extracts. In contrast, environmental conditions which resulted in a decrease in the level of extractable nitrite reductase activity produced a decline in cross-reacting material. These results indicate that the environmentally induced modulation of extractable nitrite reductase activity involves alteration of enzyme level and is not mediated by a reversible activation-inactivation of the existing enzyme.
我们之前的一篇论文（Gupta，Beevers 1983 J Exp Bot 34: 1455-1462）已经表明，豌豆（Pisum sativum cv Burpeeana）叶片中可提取亚硝酸还原酶活性的水平会受到环境干扰。在本研究中，我们使用火箭免疫电泳技术定量测定了经各种环境处理的豌豆植物提取物中的亚硝酸还原酶蛋白水平。可检测到的亚硝酸还原酶活性水平与亚硝酸还原酶交叉反应物质的水平密切相关。增强可提取亚硝酸还原酶活性的环境条件会导致提取物中产生更多的亚硝酸还原酶交叉反应物质。相反，导致可提取亚硝酸还原酶活性降低的环境条件会导致交叉反应物质减少。这些结果表明，环境诱导的可提取亚硝酸还原酶活性的调节涉及酶水平的改变，而不是通过现有酶的可逆激活-失活来介导。
