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同步小球藻中硝酸还原酶高活性和低活性形式的比较。

A comparison of the high-active and low-active form of nitrate reductase in synchronous Chlorella sorokiniana.

机构信息

Pflanzenphysiologisches Institut der Universität, Untere Karspüle 2, D-3400, Göttingen, Federal Republic of Germany.

出版信息

Planta. 1984 Jan;160(1):1-5. doi: 10.1007/BF00392458.

DOI:10.1007/BF00392458
PMID:24258364
Abstract

Chlorella sorokiniana possesses two forms of nitrate reductase (EC 1.6.6.1.). One with low activity is present in cells at the end of the light-dark cycle, the other with high activity is present after 1 h of illumination. The two forms can be distinguished by gel electrophoresis, isopycnic centrifugation, assay of the partial reactions and their sensitivity to antibodies, respectively. These differences are discussed with respect to an effect of intracellular nitrate on the activation of nitrate reductase.

摘要

盐球藻含有两种硝酸还原酶(EC 1.6.6.1.)。一种活性较低的酶存在于光暗周期结束时的细胞中,另一种活性较高的酶存在于光照 1 小时后。这两种形式可以通过凝胶电泳、等密度离心、部分反应的测定以及对抗体的敏感性来区分。这些差异与细胞内硝酸盐对硝酸还原酶激活的影响有关。

相似文献

1
A comparison of the high-active and low-active form of nitrate reductase in synchronous Chlorella sorokiniana.同步小球藻中硝酸还原酶高活性和低活性形式的比较。
Planta. 1984 Jan;160(1):1-5. doi: 10.1007/BF00392458.
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引用本文的文献

1
Evidence for a plasma-membrane-bound nitrate reductase involved in nitrate uptake of Chlorella sorokiniana.参与小球藻硝酸盐摄取的质膜结合型硝酸还原酶的证据。
Planta. 1989 May;178(1):19-24. doi: 10.1007/BF00392522.

本文引用的文献

1
[On the induction of nitrate- and nitrite-reductase in synchronized Chlorella cultures].[关于同步化小球藻培养物中硝酸盐和亚硝酸盐还原酶的诱导作用]
Planta. 1976 Jan;132(3):285-90. doi: 10.1007/BF00399728.
2
Effect of ammonium and ferricyanide on nitrate utilization by Chlorella vulgaris.铵和铁氰化物对小球藻利用硝酸盐的影响。
Planta. 1978 Jan;141(3):279-82. doi: 10.1007/BF00388344.
3
Nitrate uptake and nitrate reduction in synchronous Chlorella.同步小球藻中的硝酸盐摄取和硝酸盐还原。
Planta. 1979 Jan;146(3):287-92. doi: 10.1007/BF00387800.
4
Synthesis of Nitrate Reductase in Chlorella: II. EVIDENCE FOR SYNTHESIS IN AMMONIA-GROWN CELLS.小球藻硝酸还原酶的合成:Ⅱ. 氨培养细胞合成的证据。
Plant Physiol. 1980 May;65(5):944-8. doi: 10.1104/pp.65.5.944.
5
Synthesis of Nitrate Reductase in Chlorella: I. EVIDENCE FOR AN INACTIVE PROTEIN PRECURSOR.小球藻硝酸还原酶的合成:I. 无活性蛋白前体的证据。
Plant Physiol. 1980 May;65(5):939-43. doi: 10.1104/pp.65.5.939.
6
Light-mediated Activation of Nitrate Reductase in Synchronous Chlorella.光介导的同步小球藻中硝酸还原酶的激活
Plant Physiol. 1978 Aug;62(2):284-6. doi: 10.1104/pp.62.2.284.
7
Reduced nicotinamide adenine dinucleotide-nitrate reductase of Chlorella vulgaris. Purification, prosthetic groups, and molecular properties.普通小球藻的还原型烟酰胺腺嘌呤二核苷酸-硝酸还原酶。纯化、辅基及分子特性。
J Biol Chem. 1975 Jun 10;250(11):4120-7.