Miller C O
Department of Plant Sciences, Indiana University, Bloomington, Indiana 47401.
Proc Natl Acad Sci U S A. 1974 Feb;71(2):334-8. doi: 10.1073/pnas.71.2.334.
A cell division factor in crystalline form has been isolated from ethanolic extracts of cultured Vinca rosea L. crown gall tumor tissue. The crystalline material shows the melting point, mass spectrum, ultraviolet-light absorbancy spectra, solubilities, chromatographic migration values, ion-exchange behavior, and growth activities expected of ribosyl-trans-zeatin, that is, 6-(4-hydroxyl-3-methyl-trans-2-butenylamino)-9-beta-D- ribofuranosylpurine. This compound, which appears to be a major cell-division factor in the extracts, has also been crystallized from liquid media in which the tumor tissue had been growing and apparently was the only active compound in such media.
已从培养的长春花根癌肿瘤组织的乙醇提取物中分离出一种结晶形式的细胞分裂因子。该结晶物质具有熔点、质谱、紫外吸收光谱、溶解度、色谱迁移值、离子交换行为以及核糖基反式玉米素（即6-(4-羟基-3-甲基反式-2-丁烯基氨基)-9-β-D-呋喃核糖基嘌呤）所预期的生长活性。这种化合物似乎是提取物中的主要细胞分裂因子，它也已从肿瘤组织生长的液体培养基中结晶出来，并且显然是此类培养基中唯一的活性化合物。
