Parrish D J, Leopold A C
Department of Agronomy, University of Nebraska, Lincoln, Nebraska 68583.
Plant Physiol. 1978 Sep;62(3):470-2. doi: 10.1104/pp.62.3.470.
The initial burst of respiratory activity (Q(o) (2)) of imbibing soybean (Glycine max [L.] Merr. var. Wayne) seed tissue is cyanide-insensitive, and sensitive to salicylhydroxamate: presumptive evidence for the presence of alternate respiration. The initial O(2) consumption is also highly sensitive to propyl gallate. Soybean lipoxygenase exhibits similar characteristics of insensitivity to cyanide and sensitivity to salicylhydroxamate and to propyl gallate. The initial burst of respiration is enhanced by the addition of linoleic acid, a lipoxygenase substrate. These results indicate that the conventional tests for alternate respiration in plant tissues can be confounded by lipoxygenase; they also suggest that propyl gallate can be used to assess the possible participation of lipoxygenase in the O(2) uptake by plant tissues.
吸水大豆（Glycine max [L.] Merr. var. Wayne）种子组织呼吸活动的初始爆发（Q(o) (2)）对氰化物不敏感，而对水杨羟肟酸敏感：这是存在交替呼吸的初步证据。初始耗氧量对棓丙酯也高度敏感。大豆脂氧合酶表现出对氰化物不敏感以及对水杨羟肟酸和棓丙酯敏感的类似特征。呼吸的初始爆发通过添加脂氧合酶底物亚油酸而增强。这些结果表明，植物组织中交替呼吸的传统测试可能会因脂氧合酶而混淆；它们还表明，棓丙酯可用于评估脂氧合酶可能参与植物组织对氧气的摄取情况。
