甘薯根凝集桤生拟茎点霉（黑腐病菌）萌发孢子因子的分离与鉴定。
Isolation and Characterization of Factors in Sweet Potato Root Which Agglutinate Germinated Spores of Ceratocystis fimbriata, Black Rot Fungus.
机构信息
Institute for Biochemical Regulation, Faculty of Agriculture, Nagoya University, Chikusa, Nagoya 464, Japan.
出版信息
Plant Physiol. 1978 Nov;62(5):751-3. doi: 10.1104/pp.62.5.751.
A factor which agglutinates the germinated spores of Ceratocystis fimbriata was isolated from the sweet potato root. The factor is a glycoprotein with a molecular weight of 1.6 x 10(6) daltons and required divalent cations such as Ca(2+), Mn(2+), Ni(2+), and Mg(2+) for activity. The activity of the factor was pH-dependent. The factor also agglutinated rabbit erythrocytes and is classified as a phytohemagglutinin or lectin. The factor agglutinated germinated spores of seven strains of C. fimbriata to almost the same degree. The factor showed differential agglutinating activity toward the strains in the presence of unidentified low molecular weight factor(s) in the sweet potato root. These results support our earlier suggestion that the spore-agglutinating factors in host plants function as the determinants of specificity in some host-parasite interactions.
从甘薯根部分离出一种凝集旋孢腔菌萌发孢子的因子。该因子是一种糖蛋白，分子量为 1.6 x 10(6) 道尔顿，需要二价阳离子如 Ca(2+)、Mn(2+)、Ni(2+)和 Mg(2+)才能发挥活性。该因子的活性依赖于 pH 值。该因子还凝集兔红细胞，被归类为植物血凝素或凝集素。该因子几乎以相同的程度凝集七种旋孢腔菌的萌发孢子。该因子在甘薯根中存在不明的低分子量因子的情况下，对菌株表现出不同的凝集活性。这些结果支持了我们之前的观点，即宿主植物中的孢子凝集因子作为某些宿主-寄生虫相互作用特异性的决定因素发挥作用。
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